Phenylglyoxal modification of arginines in mammalian D-amino-acid oxidase

Astudyon D-amino-acid Oxidase

Biological role of D-amino acid oxidase and D-aspartate oxidase. Effects of D-amino acids.

D-Amino acids administered to animals are absorbed by the intestine and transported through the blood-stream to solid tissues where they are oxidized in vivo by D-amino acid oxidase and D-aspartate oxidase to produce the same compounds they do in vitro; i.e. NH3, H2O2, and the keto acid corresponding to the amino acid ingested. In the liver and kidneys of the animals, an inverse relationship ex...

Oxygen diffusion in D-amino acid oxidase

1 O2-REACTIVITY OF FLAVOPROTEINS: DYNAMIC ACCESS OF DIOXYGEN TO THE ACTIVE SITE AND ROLE OF A H RELAY SYSTEM IN D-AMINO ACID OXIDASE Jan Saam, Elena Rosini, Gianluca Molla, Klaus Schulten, Loredano Pollegioni, and Sandro Ghisla Beckman Institute, University of Illinois, 405 N. Mathews Ave., Urbana IL61801, USA; Dipartimento di Biotecnologie e Scienze Molecolari, Università degli studi dell’Insu...

Inhibition of D - Amino Acid Oxidase

In an earlier paper from this laboratory (1) there were described the characteristics of competitive inhibition resulting from the interference of certain aromatic compounds with the association of flavin adenine dinucleotide and the separated protein of n-amino acid oxidase. Included among the inhibitors were auramine, quinine, and various other quinolines and anilines. We now present our find...

Assays of D-Amino Acid Oxidase Activity

D-amino acid oxidase (DAAO) is a well-known flavoenzyme that catalyzes the oxidative FAD-dependent deamination of D-amino acids. As a result of the absolute stereoselectivity and broad substrate specificity, microbial DAAOs have been employed as industrial biocatalysts in the production of semi-synthetic cephalosporins and enantiomerically pure amino acids. Moreover, in mammals, DAAO is present...